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Fig. 1. Identification of the PSGL-1 fragment that interacts with versican G3 domain. (A) Amino acid structure of PSGL-1 showing motifs of the glycoprotein. (B) Recombinant constructs containing fragments of PSGL-1. (C) A filter membrane binding assay of recombinant PSGL-1 constructs with versican G3 domain was performed. Constructs containing amino acids 279-318 of PSGL bound to the G3 domain. The large-T antigen was used as a negative control. (D) Liquid binding activity assay using the same constructs as in C. Values shown are the mean±s.d. for the relative binding activity (n=3). **P<0.01.





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