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Fig. 6. G3 induces cell aggregation by forming multimeric forms. (A) A model of G3 multimers produced by the formation of intermolecular disulfide bonds (
) and cell aggregation mediated by G3 binding to PSGL-1 (
). (B) Culture medium was analyzed on western blots in reducing and non-reducing conditions probed with 4B6. Without DTT treatment, G3 migrated as multiple bands. In the presence of DTT, G3 migrated as a major single band. (C) U87 cells stably transfected with PSGL-1 or PSGL(279-402) were subjected to different treatments as follows: incubation with G3-containing medium; treatment with DTT before addition of G3-containing medium [+DTT (cells)] or incubation with G3-containing medium that had been treated with DTT [+DTT (G3 medium)]. When the cells were treated with DTT, a partial reduction in cell aggregation was observed. When the medium was treated with DTT, cell aggregation was completely inhibited (n=3; **P<0.01).
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