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Fig. 2. Visualization of the human chromosome-12 and chromosome-16 territories (CT12 and CT16, respectively). Gallery of 200 nm serial optical sections (every third section is shown: 0 µm, 0.6 µm, 1.2 µm, 1.8 µm, 2.4 µm, 3.0 µm) through a preadipocyte (A) and a mature adipocyte (C) nucleus after 3D-FISH with chromosome painting probes for chromosomes 12 (green) and 16 (red). A DNA counterstain is shown in blue (scale bar, 5 µm). (B,D) 3D reconstructed images of the nuclei presented in A and C, respectively, with outlines of the painted CTs and the nuclear DNA. The adipocyte nucleus displays a proximal association of one CT12 and one CT16 (C,D), whereas the preadipocyte nucleus shows no association between CT12 and CT16 (A,B).





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