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Fig. 2. Localization of endogenous PHI-1 to the trailing edge of HMVEC-L cells. (A,B) Migrating HMVEC-L cells stained for PHI-1 (green) and F-actin (red). (C) Migrating cell stained with pre-immune rabbit serum instead of anti-PHI-1 antibody, as a control. (D) Non-migrating cell stained for PHI-1 and F-actin. Magnification was 600x; bar in D, 15 µm.





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