spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 3. PHI-1 depletion by siRNA retards HeLa cell migration. (A) Total cell lysates (20 µg per lane) from cells transfected with buffer, {alpha}4 siRNA or PHI-1 siRNA#2 resolved by SDS-PAGE and blotted for PHI-1, {alpha}4 and PP1. `C' is a band present in PHI-1 immunoblots that serves as loading control. (B) Quantitation of the distance of migration 17 hours after scraping of cells transfected with buffer, {alpha}4 siRNA or PHI-1 siRNA#2 (*P<0.05). (C) Kinetics of cell migration under PHI-1 depletion. Same as B, except that cell migration was followed for a total of 41 hours after scraping (circles represent control cells and triangles represent PHI-1-knocked-down cells).





Right arrow Return to article