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Fig. 4. PHI-1 depletion using siRNAs results in extremely elongated HeLa cells that fail to retract. (A) Time-lapse phase-contrast photomicroscopy of control cell exhibiting two cycles of protrusions and retractions (white arrows) in a 3-hour period. Original magnification was 160x; bar, 35 µm. (B) Time-lapse phase-contrast photomicroscopy of a cell depleted of PHI-1 extending along the direction of the double-headed arrow with no retraction events in 5-hour period. Original magnification was 100x; bar, 40 µm. See Movie 1 and 2 in supplementary material.





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