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Fig. 6. PHI-1 knockdown by siRNA inhibits lamellipodium retraction but not protrusion in HUVECs. (A) The surface areas of replated cells were compared by overlaying binary images of cells acquired every 30 seconds. The absolute difference between the two surfaces ({Delta}) was used to determine protrusion (P) and retraction (R) events. (B) The number of protrusion events (black bars) between control and PHI-1 knockdown HUVECs was not significantly different. However, the number of retraction events in PHI-1-knockdown cells was significantly reduced compared with control cells (29±0.3 vs 44±4.4, respectively; *P<0.05, n=3).





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