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Fig. 7. PHI-1 knockdown by siRNA does not affect stress fibers or the phosphorylation state of selected cytoskeleton and focal-adhesion proteins. (A) Control HeLa cells stained for actin stress fibers. (B) PHI-1-depleted HeLa cells stained for actin stress fibers. Magnifications for A and B were 600x; bar for A and B, 25 µm. (C) Immunoblots of total cell lysates (20 µg per lane) from control and PHI-1-depleted HeLa cells were resolved by SDS-PAGE and blotted for PHI-1, phosphorylated and total cofilin, phosphorylated and total ERK, phosphorylated (P) and total (T) ERM, FAK phosphorylated on Ser722, phosphorylated MLC, and paxilin. `C' is a band present in PHI-1 immunoblots that serves as loading control.





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