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Fig. 5. Type IV collagen, but not laminin, is absent from the BM of Hsp47–/– embryos at 9.5 dpc, as determined by immunoelectron microscopy. (A-D) Cryosections of wild-type and Hsp47–/– embryos that include the BM and neighbouring epithelial cells were first incubated with antibodies against type IV collagen (A,B) or laminin (C,D) and then with secondary antibodies conjugated with gold particles. Ultrathin sections were analysed by electron microscopy. Arrows indicate BM regions. Note that the staining of the BM with gold particles is observed in panels A, C and D but not in panel B. EP, epithelial cells; ME, mesenchyme. Bar, 0.1 µm.
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