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Fig. 8. DNA isolated from Hsp47–/– embryos at 10.5 dpc, but not at 9.5 dpc, shows the ladder pattern that is typical of apoptosis. DNA extracted from wild-type and Hsp47–/– embryos at 9.5 and 10.5 dpc was analysed by agarose gel electrophoresis. The DNA ladder was observed only in Hsp47–/– embryos at 10.5 dpc (right side), although none of the embryos displayed DNA fragmentation at 9.5 dpc (left side). Size markers: A, lambda phage DNA digested with HindIII; B, {varphi}x174 DNA digested with HaeIII.





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