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Fig. 2. Effect of electroporation on intracellular Ca²⁺ stores in HeLa cells. Graphs show normalized fluorescence (F/F_O(cytoplasm). (A) Cells in 2 mM extracellular Ca²⁺ were loaded with fluo-4AM and stimulated with 50 µM histamine at t₁₀₀ seconds. (B) Cells in 2 mM EGTA were incubated for 15 minutes and stimulated with 50 µM histamine at t₁₀₀ seconds. (C) Cells in 2 mM EGTA were electroporated with fluo-4 and stimulated with 50 µM histamine at t₁₀₀ seconds. (D) Cells electroporated in 2 mM EGTA were stimulated with 2.1 mM Ca²⁺ at t₃₀ seconds and then stimulated with 50 µM histamine t₁₀₀₀ seconds. Arrows indicate addition of 50 µM histamine.

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