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Fig. 6. Ca²⁺-dependent nuclear translocation of 6ROX-CaM in glial cells (A) Glial cell electroporated with 6ROX-CaM and fluo-4 in 2 mM EGTA (t₀). Cells were stimulated with 2.1 mM Ca²⁺ at t₅₀. t_1/2 of the translocation of 6ROX-CaM into the nucleus was 40 seconds. At t₃₀₀ seconds, the concentration of 6ROX-CaM associated with nucleoli and in the nucleoplasm had increased 1.5-fold and 1.4-fold, respectively. Nuclear translocation of 6ROX-CaM and fluo-4 was measured at 0, 50, 150, 300, 600 and 900 seconds (t₀ to t₉₀₀) and the respective data are shown in (B and C) Colored boxes indicate areas that have been measured for nuclear translocation of 6ROX-CaM and fluo-4. (B) Time course of normalized 6ROX-CaM fluorescence (F/F_(O)cytoplasm) in the () nucleolus, () nucleoplasm and (^*) cytoplasm. The arrow indicates the addition of 2.1 mM Ca²⁺. (C) Time course of intracellular free Ca²⁺ concentration changes monitored by fluo-4 normalized fluorescence (F/F_(O)cytoplasm) in the same area of the cell as 6ROX-CaM in B.

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