QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 7. Ca²⁺-dependent nuclear translocation of 6ROX-CaM in rat cortical neurons. (A) Neurons electroporated with 6ROX-CaM and fluo-4 in 2 mM EGTA (first image, t₀). The cell was stimulated with 2.1 mM Ca²⁺ (t₅₀). t_1/2 of the translocation of 6ROX-CaM into the nucleus was 90 seconds. At t₃₀₀ seconds, the concentration of 6ROX-CaM associated in the nucleoplasm had increased 1.7-fold. Nuclear translocation of 6ROX-CaM and fluo-4 was measured at 0, 50, 270 and 400 seconds (t₀ to t₄₀₀) and the respective data are shown in (B and C) Colored boxes indicate areas that have been measured for nuclear translocation of 6ROX-CaM and fluo-4. (B) Time course of the F_np/F_cytoplasm ratio of 6ROX-CaM and (C) and of the normalized fluorescence (F/F_(O)cytoplasm) of fluo-4 in a representative record. Arrows indicate 2.1 mM Ca²⁺ addition.

Return to article