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Fig. 9. Inhibition of nuclear translocation by WGA. (A-C) Inhibition of Ca²⁺-dependent nuclear translocation of 6ROX-CaM in HeLa, neuronal and glial cells by WGA. HeLa cells (A) were electroporated with 6ROX-CaM and 20 µg/ml WGA in 2 mM EGTA (t₀). The cells was stimulated with 2.1 mM Ca²⁺ at t₆₀. A further image, showing inhibition of nuclear translocation, was taken at t₂₅₀. Neurons (B) were electroporated with 6ROX-CaM and 20 µg/ml WGA in 2 mM EGTA (t₀). The cells were stimulated with 2.1 mM Ca²⁺ at t₃₆. A further image, showing inhibition of nuclear translocation, was taken at t₁₅₀. Glial cells (C) were electroporated with 6ROX-CaM and 20 µg/ml WGA in 2 mM EGTA (t₀). The cells were stimulated with 2.1 mM Ca²⁺ at t₄₃. A further image, showing inhibition of nuclear translocation, was taken at t₁₇₉. (D) HeLa cell electroporated with 6ROX-CaM1234 and 20 µg/ml WGA in 2 mM EGTA (t₀). The cell was stimulated with 2.1 mM Ca²⁺ at t₇₉. A further image, showing inhibition of nuclear translocation, was taken at t₂₀₀.

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