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Fig. 3. H3V associates with the spindle during mitosis. (A-D) PFJEL4, a cell line expressing H3V-YFP (green), was fixed and stained with an antibody that recognizes ß-tubulin (red). This antibody detects both the cytoskeleton and the mitotic spindle, and thus the outline of each cell is seen. DNA (blue) was visualized with DAPI. (A) At interphase, no spindle is observed and H3V-YFP is predominantly at the nuclear periphery. (B) In early mitosis, the spindle is visualized and H3V-YFP appears arrayed across it. (C,D) H3V-YFP continues its association with the spindle as it is pulled toward the poles. Bar, 2 µm.





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