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Fig. 6. H3V is enriched at telomeres. (A) Chromatin immunoprecipitation was carried out on procyclic wild-type cells (29.13) or cells expressing H3V-YFP (PFJEL4) or H3-YFP (PFJEL7). In each series, chromatin was immunoprecipitated with either a non-specific ( ${alpha}$ 117) or a specific antibody ( ${alpha}$ GFP). Immunoprecipitated DNA was analyzed by slot blots hybridized with probes specific for telomeric repeats (TEL), mini-chromosome 177-bp repeats (MC177), or 5SDNA. Note that the input control corresponds to 10% of total. ChIPs were performed two to four times and a representative slot blot is shown for each set. (B) A quantitative presentation of the data shown in A and from one to three other independent experiments reveals the average percentage of input material present in control ( ${alpha}$ 117) and test ( ${alpha}$ GFP) ChIPs. (C) Relative to major H3, H3V is enriched at telomeres and is found at reduced levels at two other genomic loci. To determine these ratios, the background signal value (i.e. the material immunoprecipitated by the non-specific antibody) was subtracted from ${alpha}$ GFP signal value to generate a specific signal value; the specific signal value of H3V-YFP divided by the specific signal value of H3-YFP equals the relative enrichment.

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