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Fig. 7. Silencing of an expression site upon differentiation to the procyclic form is unaffected by loss of H3V. (A) Schematic diagram of telomere (zig-zag) proximal portion of the 221 expression site and the 221 expression site in which either GFP-BLE cassette or T7 promoter driven GFP-BLE was inserted. (B) Comparison of the GFP fluorescence intensity from the wild type (single marker), wild type + GFP (BFJEL36), wild type + T7-GFP (BFJEL37) and htv{Delta}/htv{Delta} (BFJEL25), htv{Delta}/htv{Delta} + GFP (BFJEL38) and htv{Delta}/htv{Delta} + T7-GFP (BFJEL39) bloodstream-form cell lines and the same cell lines following two weeks differentiation to the procyclic form.





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