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Fig. 5. Epitope tags reduce the destruction of Aur-A in vitro. (A) Radiolabeled IVT products were prepared for the indicated proteins using 0.2 µg DNA templates: (1) Xenopus Aur-A; (2) N-terminal AU1-tagged Xenopus Aur-A; (3) human Aur-A; (4) N-terminal AU1-tagged human Aur-A; (5) N-terminal GFP-tagged human Aur-A; and (6) C-terminal GFP-tagged human Aur-A. (B) IVT products were incubated with G1-phase HeLa-cell lysates. Samples were taken at the indicated times and monitored by SDS-PAGE followed by autoradiography. (C) Destruction assays were performed in Xenopus egg extracts as described in Fig. 3A.





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