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Fig. 2. SU6656 inhibits activated FGFR-mediated tyrosine phosphorylation of hSpry2. (A) Inhibition of activated FGFR-mediated tyrosine phosphorylation of hSpry2 by SU6656. hSpry2 was co-transfected with wild-type FGFR2 into 293T cells. Cells were pre-treated with various concentrations of SU6656 for 1 hour before FGF2 stimulation (30 minutes). hSpry2 was immunoprecipitated and then immunoblotted with anti-hSpry2 antibody or anti-phosphotyrosine antibodies (IP:hSpry2). Antibodies against SFKs (anti-SRC2) and active SFKs (anti-SRC pY416) were used to examine SFK expression and activity. (B) Effect of 20uM SU6656 on overexpressed FGFR2-induced tyrosine phosphorylation profile. 293T cells were transfected with FGFR2 and were either pre-treated with 20 µM SU6656 or DMSO (control) for 1 hour prior to FGF2 stimulation for 30 minutes. FGFR2 was immunoprecipitated (IP:FGFR2) and immunoblotted with both and anti-phosphotyrosine antibody anti-FGFR2 antibodies (IB:pY and IB:FGFR2, respectively).





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