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Fig. 5. hSpry2 is a direct substrate for SRC kinase. Five micrograms of the N-terminal region of hSpry2WT (amino acids 1-172) was used as a substrate for an in-vitro kinase assay. Reaction conditions included either the absence or presence of 100 µM ATP and/or 200ng of active SRC. Tyrosine-phosphorylation was confirmed using anti-phosphotyrosine antibodies. Equal substrate concentration was confirmed by anti-hSpry2 antibody.





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