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Fig. 1. Replication kinetics in Xenopus egg extract. (A) Sperm nuclei were incubated at 12 ng DNA µl-1 in interphase Xenopus egg extract supplemented with [{alpha}-32P]dATP. At the indicated times, total DNA synthesis was measured. (B) Sperm nuclei were incubated in extract at 12 ng DNA µl-1. At the indicated times, samples were pulse labelled with [{alpha}-32P]dATP for 2 minutes. DNA was separated on an alkaline agarose gel and autoradiographed. The migration of end-labelled {lambda}-phage HindIII-digested DNA is also shown.





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