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Fig. 5. The intra-S-phase checkpoint reduces Cdc45 and PCNA on chromatin. (A) Sperm nuclei were incubated at 10 ng DNA µl-1 in interphase extract supplemented with various concentrations of aphidicolin minus (left) or plus (right) 5 mM caffeine. Chromatin was isolated and immunoblotted for XCdc45 and Rad17. (B) Sperm nuclei were incubated at 10 ng DNA µl-1 in interphase extract supplemented with different combinations of 15 µM aphidicolin and/or 5 mM caffeine. At the indicated times, chromatin was isolated and immunoblotted for XCdc45 and PCNA.





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