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Fig. 9. Fur4p and Sur7p do not localize with Pma1p in plasma membranes of living cells. Simultaneous localizations of Fur4GFP/Pma1mRFP (two left columns) and Pma1GFP/Sur7mRFP (two right columns) were performed. Fluorescence signals from transversal confocal sections (first and third columns from the left), and surface confocal sections (second and fourth columns) are shown. GFP (A-D) and RFP (E-H) fluorescence channels and a merged image (I-L) are shown. The fluorescence-intensity profiles along the cell surface (outside the dashed arrows in I,K) and through the surface section (between the lines in J,L) were plotted in M,O and N,P, respectively. The curves were smoothed by mean filter to reduce the noise and normalized to the same maximum value. Bar, 10 µm.





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