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Fig. 2. Inhibition of HBP expression inhibits histone gene expression. Asynchronous HeLa cells were transfected with either the U6pHP1, U6pHP2, U6pHP3, U6pcHP or U6pT cassette and RNA samples were prepared 24 hours or 48 hours after transfection and analysed by northern blotting for HBP mRNA and histone mRNA, and for 18S rRNA as loading control. (A) Effect of transfection with U6pT, U6pHP1, U6pHP2, U6pHP3 or U6pcHP on HBP and histone H3 expression 24 hours after transfection. mRNA levels were standardised with respect to 18S rRNA and expressed as the percentage of mRNA in the cells transfected with U6pT (=100%). (B) Effect of mock transfection (no DNA) and transfection with U6pHP1 or U6pT on histone H1, H2A, H2B, H3 and H4 mRNA levels 24 hours and 48 hours after transfection. (C) mRNA levels from B standardised with respect to 18S rRNA and expressed as a percentage of mRNA in the `no DNA' control (=100%) at 24 hours and at 48 hours after transfection.





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