QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 3. HBP expression is essential for cell proliferation. (A) Schematic drawing showing the structure of the pEGFP-C3 derivatives used to determine the effect of HBP RNAi on cell proliferation. The cassettes expressing shRNAs were inserted in the unique MluI restriction site located outside the sequence elements involved in the expression of EGFP (expressed from the pCMV promoter and using the SV40 polyadenylation signal) or the neomycin phosphotransferase gene (expressed from the pSV40 promoter and using the Herpes simplex thymidine kinase (HSV TK) polyadenylation signal). Indicated are the U6 RNA promoter and terminator (U6P, TTTTT) flanking the inverted repeat coding for the shRNA. (B) HeLa cells were transfected with pEGFP-U6pT, pEGFPU6pcHP, pEGFP-U6pHP1, pEGFP-U6pHP2 or pEGFP-U6pHP3, or mock treated without DNA. Subsequently G418 was added and cells were analysed as described in Materials and Methods. Shown are cells prior to addition of G418 (0 days) and after addition of G418 (3 days and 7 days).

Return to article