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Fig. 4. Inhibition of HBP expression inhibits cell cycle progression. HeLa cells were arrested by treatment with nocodazole and subsequently released. One hour after release, cells were transfected with either pGEM-U6pT or pGEM-U6pHP1 or mock transfected without DNA. Samples were prepared for FACS analysis by staining with propidium iodide at the indicated times after release. (A) FACS profiles 8, 16 and 26 hours after release from nocodazole block. The boundaries that define cells with sub-G1-, G0/G1-, S- and G2/M-phase DNA content were determined using asynchronous HeLa cells and are shown in the 8 hours/noDNA samples. Identical settings were used for the analysis of the other samples. (B) Cell cycle distribution. Shown is the percentage of cells with sub-G1-, G0/G1-, S- and G2/M-phase DNA content. Results are representative of two independent experiments.





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