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Fig. 2. Scrib domain localization in scrib mutant neuroblasts and epithelia of stage 15 embryos. Left column, metaphase neuroblasts; middle column, interphase neuroblasts; right column, interphase epithelial cells; apical up for all images. The genotype is indicated to the left of each row; antibodies used to detect endogenous Scrib (A) or Scrib:myc (B-H) are indicated to the right of each row. (A) Wild-type embryos showing endogenous Scrib protein localization. (B-H) scrib1/scrib1 embryos showing localization of the indicated Scrib:myc proteins detected with Myc antibody. Each UAS-scrib:myc transgene was expressed using worniu-gal4 (for the neuroblasts) or scabrous-gal4 (for the epithelial). Insets show the same neuroblast stained for {alpha}-tubulin (green) as a cell cycle indicator and Scrib (A) or Scrib:myc (B-H) protein (red). Scrib domain abbreviations are given in Fig. 1. (I) Quantification of endogenous Scrib and Scrib domain localization in stage 15 metaphase neuroblasts. Apical enrichment, black bar; uniform cortical localization, crosshatched red bar; cytoplasmic localization, blue bar. Scrib domain abbreviations are given in Fig. 1.





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