|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 7. Src S17 phosphorylation is required for neurite outgrowth induced by cAMP in PC12 cells. (A) ERK mediates neurite outgrowth induced by NGF and dbcAMP, but SFKs are involved only in neurite outgrowth induced by cAMP. PC12 cells were incubated with NGF or dibutyryl cAMP (dbcAMP) for 24 hours in the presence or absence of PD98059 or PP2, and neurite outgrowth was evaluated microscopically. PD98059 blocked neurite outgrowth induced by both NGF and dbcAMP, whereas PP2 only blocked outgrowth induced by dbcAMP. (B) Bar graph of mean percentage±s.e. of cells bearing neurite outgrowths when treated as described in A (n=3-5). (C) Src S17A blocks dbcAMP-induced neurite outgrowth, but not NGF-induced neurite outgrowth. PC12 cells were cotransfected with either pcDNA3 (vector) or Src S17A and with EGFP as a marker for transfected cells. The cells were incubated with NGF or dbcAMP for 24 hours. Neurite outgrowth was observed under fluorescence microscopy to identify transfected cells. (D) Bar graph of mean percentage±s.e. of EGFP-positive cells bearing neurite outgrowths when treated as described in C (n=3-5).
|
