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Fig. 2. Indirect immunofluorescence analysis of oocytes synthesizing nuclear lamins. Cryostat sections (A-E) and nuclear envelope spread preparations (F-L) are shown. The type of RNA injected is indicated at the margins of each panel. n.i., non-injected control oocytes. Oocyte lamin LIII was detected with mAb NUC195 (A) or mAb L6-5D5 (F), Flag-A, Flag-B1 and Flag-LIII with mAb M2 (B-E,H,K), lamin B1 and non-injected control envelopes were reacted with mAb L7-4A2 (I,L); myc-LIII with mAb 9E10 (G); lamin B2 with mAb L7-8C6 (J). Cy3-conjugated goat anti-mouse IgG was used as a secondary antibody. Brighter staining along folds of the nuclear envelope in F-H, K is due to folding of the nuclear envelope during the spreading procedure. Bars, 20 µm.





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