spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 3. Electron microscopy of lamin-induced intranuclear membrane structures. (A-E) EM sections of isolated oocyte nuclei are shown. Oocytes were injected with RNA encoding lamin B2 (A-D) or lamin B2-SaaX (E). Nuclei were isolated and processed for electron microscopy. Sections were stained with uranyl acetate and lead citrate (A-C,E) or processed for pre-embedding immunoelectron microscopy (D). Lamin B2 was detected with mAb L7-8C6 and nanogold-coated secondary antibody. Arrows in A indicate membrane arrays attached to the nuclear envelope. Arrows in D indicate gold particles decorating the outer membrane cisternae of a lamin B2-induced membrane array. C, cytoplasm; NC, nucleoplasm; NE, nuclear envelope; NPC, nuclear pore complex. Bar, 5 µm (A); 1 µm (B,E); and 0.5 µm (C,D).





Right arrow Return to article