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Fig. 5. NLS-MT-GFP-N-Ras chimeras induce formation of intranuclear membrane arrays in HeLa cells. (A-D) HeLa cells transiently transfected with NLS-MT-GFP-N-Ras were processed for EM 24 hours after transfection. Sections were stained with uranyl acetate and lead citrate. Note the formation of stacked membrane cisternae aligned with the nuclear envelope as well as the formation of membrane arrays within the nucleoplasm. NE, nuclear envelope; PM, plasma membranes. Bar, 5 µm (A); 1 µm (B-D).





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