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Fig. 2. Characterization of GFP-lamin fusion proteins. Total protein of COS-7 cells (lanes 1-12), Xenopus A6 cells (lanes 13, 14) and zebrafish embryos (lane 15) expressing the GFP-lamin fusion proteins listed in Fig. 1 were separated by SDS-PAGE and immunoblotted with GFP antibodies. COS-7 and A6 cells were transfected and zebrafish embryos were microinjected with in vitro synthesized lamin mRNA at the 2-4 cell stage. Embryos were analyzed 24 hours post fertilization. Lane 1, non transfected cells; lane 2, human lamin A (hA); lane 3, Drosophila lamin Dm0 (dDm0); lane 4, Drosophila lamin C (dC); lanes 5 and 11, Drosophila lamin C-CxxM (dC_CxxM); lane 6, Xenopus lamin B2 (xB2); lane 7, Xenopus lamin B2- ${Delta}$ N1 (xB2- ${Delta}$ N1); lane 8, Xenopus lamin B2- ${Delta}$ N2 (xB2- ${Delta}$ N2); lane 9, Xenopus lamin B2 mutant B2-SxxM (xB2_SxxM); lane 12, Drosophila lamin C_CxxM- ${Delta}$ N1 (dC_CxxM- ${Delta}$ N1); lane 13, Xenopus lamin B2- ${Delta}$ N3 (xB2- ${Delta}$ N3); lanes 14 and 15, zebrafish lamin B2- ${Delta}$ N3 (zB2- ${Delta}$ N3). Molecular masses of reference proteins (lanes M) are marked and are from top to bottom: 116, 97, 66 and 45 kDa.

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