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Fig. 4. Electron microscopy of ultrathin sections through microinjected Xenopus A6 cells. (A-E) A6 cells overexpressing Xenopus lamin B2 (A,D), human lamin A (B), Drosophila lamin Dm0 (C) and lamin C (E). Note the highly lobulated nuclear envelopes (A-C), small (B,C, arrows) and large (D, arrow and inset) intranuclear membrane assemblies. (D, inset) A dot-like membrane assembly is shown at higher magnification. (E) Drosophila lamin C forms membrane-free electron-dense aggregates at the nuclear envelope (arrow) and in the nucleoplasm (arrowhead) that have a banded substructure when sectioned parallel to the longitudinal axis of the lamin aggregates (inset). cy, cytoplasm; no, nucleolus; nu, nucleoplasm. Bars 1 µm (A-E); 500 nm (insets in D,E).





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