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Fig. 5. Intranuclear membranes are formed in cells overexpressing wild-type lamins. (A-I) High magnification electron micrographs of ultrathin sections through microinjected Xenopus A6 cells expressing Xenopus lamin B2 (A,F,H), human lamin A (C-E,I) and Drosophila lamin Dm0 (B,G). Intranuclear membranes form flat cisternae that are separated from the inner nuclear membrane by a 15-25 nm-thick layer similar in electron density to the lamina (B, arrow). Examples where only a few locally restricted intranuclear membranes were found are shown in A and B, and a nuclear envelope nearly completely bordered by an intranuclear membrane cisterna in C. (D) Nuclear envelope bordered in neighboring areas by one (arrowhead 1), two (arrowhead 2) and three (arrowhead 3) membrane cisternae. (E) Part of a highly lobulated nucleus showing an area where the nucleoplasm is nearly excluded between the multi-membrane-layered nuclear membranes. (F-H) Lobulated intranuclear membrane cisternae of increasing complexity. (I) Irregular vesicle-like intranuclear membranes. Nuclear pore complexes are indicated by arrowheads in A-C, I. cy, cytoplasm; ne, areas of the nuclear envelope with an unaltered morphology; nu, nucleoplasm. Bars, 500 nm.
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