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Fig. 8. Expression of GFP-lamin fusion proteins in COS-7 cells and zebrafish embryos. (A-E) Electron micrographs of ultrathin sections through transfected (A) and microinjected COS-7 cells (D,E) and zebrafish embryos at 9 (B) and 36 (C) hours post fertilization. Cells express Xenopus lamin B2-N3 (A) and human lamin A (D,E). Zebrafish embryos were microinjected at the 2-4 cell stage with mRNA encoding zebrafish lamin B2 mutant zB2-N3 (B,C). Multi-layered nuclear membranes (A,C) and nuclear lobulations (B, arrow) were seen in cells overexpressing this lamin B2 mutant. The arrowhead in B indicates an unaltered area of the nuclear envelope containing pore complexes. (D,E) COS-7 cells expressing human lamin A possess an electron-dense layer of irregular thickness underneath the inner nuclear membrane (D) or spherical aggregates attached to the inner nuclear membrane (E). Intranuclear membranes are attached to some spherical aggregates (E, arrow). cy, cytoplasm; nu, nucleoplasm. Bars, 500 nm (A,C-E); 1 µm (B).

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