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and BAF transiently localize to telomeres and specific regions on chromatin during nuclear assemblyFiles in this Data Supplement:
Movie 1. Movie of projections of confocal z-stacks showing dynamics of coexpressed YFP-LAP2a and CFP-histone 2B in HeLa cells during nuclear assembly. False colors (green for YFP and red for CFP) are used in the merged images. Stacks were acquired every 10-180 seconds as indicated by the time scale at the upper left corner. Confocal z-stacks consist of ten 1.3-mm-thick optical sections. Frame rate, 4 fps. Arrows indicate initial LAP2a chromatin-association sites at telomeric structures. Still frames of a selected z-section are shown in Fig. 1B.
Movie 2. Movie of projections of confocal z-stacks showing dynamics of coexpressed YFP-LAP2a and CFP-LAP2b in HeLa cells during nuclear assembly. False colors (green for YFP and red for CFP) are used in the merged images. Stacks were acquired every 10-180 seconds as indicated by the time scale at the upper left corner. Confocal z -stacks consist of eleven 1.5-mm-thick optical sections. Frame rate, 4 fps. Still frames of a selected z-section are shown in Fig. 4.
Movie 3. Movie of projections of confocal z-stacks showing dynamics of coexpressed YFP-LAP2a and CFP-LBR in HeLa cells during nuclear assembly. False colors (green for YFP and red for CFP) are used in the merged images. Stacks were acquired every 10-180 seconds as indicated by the time scale at the upper left corner. Confocal z-stacks consist of eleven 1.5-mm-thick optical sections. Frame rate, 4 fps. Still frames of a selected z-section are shown in Fig. 4.
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