spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 2. LAP2{alpha} associates with telomeres in stable structures. (A) HeLa cells were fixed and processed for immunofluorescence using antibodies to LAP2{alpha} (green) and TRF2 (red). Arrows indicate areas shown at higher magnifications in inserts. (B) Mitotic HeLa cells were incubated for 10 minutes (a-c) or 20 minutes (d,e) in hypotonic buffer, followed by preparation of chromosome spreads. Samples were processed for immunofluorescence microscopy using monoclonal (a) or polyclonal (b-d) antibodies to LAP2{alpha} or monoclonal antibody to LAP2ß (e) and the Hoechst DNA stain. Confocal images are shown. (C) FRAP analyses. The boxed areas were bleached to background levels (0 s) and recovery monitored after indicated time points. Fluorescence recovery after 8 seconds as a percentage of the prebleach intensity was corrected for intensity changes in unbleached zones and plotted. Bars, 10 µm.





Right arrow Return to article