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Fig. 3. LAP2 ${alpha}$ dynamics in relation to lamins. (A) HeLa cells stably expressing YFP-LAP2 ${alpha}$ were transiently transfected with constructs encoding either CFP-lamin C or CFP-lamin B1 and analyzed by live cell imaging. Confocal images of cells in interphase and various stages during anaphase to G1 phase progression are shown. Time points show elapsed time relative to first image. Arrowheads denote LAP2 ${alpha}$ in the inner core region; arrows, lamin structures. (B) Immunofluorescence microscopy of fixed GFP-LAP2 ${alpha}$ -expressing cells using antibodies to lamins A/C. Bars, 10 µm.

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