spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 10. Taxol displaces tau from microtubules in vitro. CHO cells transfected with PcDNA3 (lanes 1 and 1'), wild-type tau (lanes 2 and 2') or GFP-tau (lanes 3 and 3') were homogenized and the supernatants were subjected to conditions allowing the polymerization of tubulin in vitro in the absence (lanes 1,2,3) or presence (lanes 1',2',3') of taxol. Microtubule pellets were examined by SDS-PAGE as described in Materials and Methods. Tau protein and GFP-tau were detected with a polyclonal antibody against the C-terminus of tau (A). The same blots were developed with antibody against {alpha}-tubulin (B).





Right arrow Return to article