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Fig. 2. GFP-tau uniformly labels individual MTs in Xenopus embryo neurons regardless of their location along the axon. (A) Fluorescence image of a neuron expressing GFP-tau. (B,C) Higher-magnification views of the growth cone region (B) and the proximal axon (C) taken from areas marked by rectangles in A. Individual MTs can be resolved in both areas, allowing for direct comparison of tau binding to MTs in different axonal regions. Bar, 10 µm. (D) Quantitative analysis of tau binding to individual MTs at the growth cone and at the proximal axonal segment. Axons chosen for analysis were 150-250 µm in length. The fluorescence intensity of individual MTs was measured at the growth cone region and at the middle axonal segment and, after background subtraction, scaled to adjust for the different levels of tau expression in different neurons. Data are presented as means±s.e.m. Data from 40 MTs in five different neurons.
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