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Fig. 3. Binding of tau to individual MTs depends on MT curvature. (A) Fluorescence micrograph of a fibroblast expressing GFP-tau. Individual MTs can be detected at the lamella region. The boxed regions are shown below at a higher magnification. (B-D) A series of fluorescence images of the lamella of the fibroblast. Images were taken at 3-second intervals. Sites of higher MT curvature (arrows) display higher levels of fluorescence intensity. (E) Fluorescence micrograph of the axonal shaft with a flat growth cone-like protrusion at the lateral edge. The boxed region of the image on the left is shown at higher magnification on the right as a series of images acquired at 3-second intervals. (F) Quantitative analysis of the data for GFP-tau-labeled MTs in fibroblasts (open triangles), GFP-tau-labeled MTs in neurons (stars), and GFP-tubulin-decorated MTs in fibroblasts (filled triangles). Each datum point represents background-subtracted fluorescence intensity of MT at the site of high curvature (I) normalized to that at the straight segment of the same MT (I_o), versus the radius of MT curvature (R_MT). Data for the cells expressing GFP-tau are grouped together and fitted with a single exponential.

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