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Fig. 9. Detergent extraction of GFP-tau-expressing cells. Fluorescence images of fibroblasts (A,B) and a neuron (C) expressing GFP-tau before (top images) and after (bottom images) extraction in a buffer containing 10 µM taxol. The time after the onset of extraction is shown in the lower-right corner of each panel. Individual MTs (arrows in A) rapidly lose associated GFP-tau. In contrast, GFP-tau remains associated with MT bundles (arrowheads in B,C) for as long as 30 minutes after extraction. Bars, 10 µm.





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