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Fig. 2. Localization of histone modifications in quiescent lymphocytes and 3T3 fibroblasts. Quiescent lymphocytes from mouse spleen (a-g) and 3T3 cells (h-m) stained with antibodies against H4K12Ac, H3K9Me2, H3K9Me3, and Hoechst as indicated. Overlays double-stained with antibodies against H4K12Ac and Hoechst (c), H3K9Me2 and Hoechst (d,j) and H3K9Me3 and Hoechst (m). The positions of line profiles shown on panels a and b are indicated by the broken lines on panels c and d, respectively. Vertical arrows on panels a and b denote the approximate locations of the apocentric zone where H4K12Ac is absent, and H3K9Me2 maximal.





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