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Fig. 3. Histone modifications in reactivated lymphocytes. (a,b) Nuclear proteins from resting (Con A -) and Con A-activated (Con A +) spleen lymphocytes were separated by SDS-PAGE and stained (lanes 1, 2, 5, and 6) or probed for H4AcK12 (lanes 3, 4) and H3K9Me2 (lanes 7, 8). (c,d) Con A-activated lymphocytes from mouse spleen double-stained for H4K12Ac and Hoechst (c) and H3K9Me2 and Hoechst (d).





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