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Fig. 6. Reciprocal accumulation of macroH2A and HP1 ${alpha}$ in NIH/3T3 cells. (a,b) NIH/3T3 cells transfected with expression vectors encoding express-macroH2A (panels 1-9), express-macroH2A_1-140 (panels 10-11), and co-expressing macroH2A and express-H2A.l (12,13), and macroH2A and express-H2A.X (14, 15). Slides were stained with ${alpha}$ -express (1,4,7,10,13,15), ${alpha}$ -HP1 ${alpha}$ (3,6), ${alpha}$ -HP1ß (9), ${alpha}$ -macroH2A (12,14) and Hoechst 33258 (2,5,8). Cells in panels 4-15 were incubated for 48 hours with 5 mM sodium butyrate.

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