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Fig. 4. Suppression of the echinocandin-hypersensensitive growth phenotype of the ehs2-1 mutant by overexpression of rho1+. MS38 (rgf3+) was transformed with pREP3X (empty vector) and GI 1 (ehs2-1/rgf3) was transformed with pREP3X-rho1 (rho1+), pREP3X-rho2 (rho2+), pREP3X-rho3 (rho3+), pREP3X-rho4 (rho4+), pREP3X-rho5 (rho5+), pREP3X-cdc42 (cdc42+) and pREP3X (empty vector). Transformants were streaked onto MM, MM plus thiamine, MM plus 1.5 µg ml-1 echinocandin and MM plus thiamine and 1.5 µg ml-1 echinocandin plates, and incubated at 32°C for 4 days.





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