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Fig. 3. PKC activity assays. Innervated and noninnervated PM and MA muscle fiber cultures incubated in control medium or medium containing 100 µM ryanodine were assayed for PKC activity. Results are expressed relative to PKC activities in noninnervated PM and MA muscle fibers incubated in control medium. PKC activities in PM muscle fibers in the presence of either innervation or 100 µM ryanodine were not significantly different. PM muscle fibers in the presence of both innervation and ryanodine had significantly less PKC activity (P<0.05). MA muscle fibers in the presence of innervation displayed significantly lower PKC activities (P<0.05). Bars represent means±s.e.m.; n=5.