spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 6. Electromobility shift and supershift assays of the slow MyHC2 NFAT binding site. (A) Nuclear extracts from innervated (SC) and noninnervated PM (lanes 2-5) and MA (lanes 6-9) muscle fibers incubated in control medium or medium containing 100 µM ryanodine (Ry) were incubated with the NFAT binding site oligonucleotide probe. Protein-DNA complexes were resolved in 5% nondenaturing polyacrylamide gel. A specific protein-DNA complex (arrowhead) formed from extracts of innervated PM muscle fibers incubated in medium containing ryanodine (lane 5) and from extracts of innervated MA muscle fibers (lanes 8 and 9). Lane 1 contained no nuclear extract. A faster migrating protein-DNA complex (asterisk) of unknown composition was present in each lane. (B) Inclusion of an anti-NFATc1 antibody in the binding reaction resulted in a supershifted protein-DNA complex (arrow). An anti-MEF2a antibody did not yield a supershifted complex.





Right arrow Return to article