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Fig. 7. Electromobility shift and supershift assays of the slow MyHC2 MEF2 binding site. (A) Nuclear extracts from innervated (SC) and noninnervated PM and MA muscle fibers were incubated with the MEF2 binding site oligonucleotide. Lane 1 contained no nuclear extract. Lanes 2-5 contained PM nuclear extract, and lanes 6-9 contained MA nuclear extracts. A protein-DNA complex (arrowhead) was identified in lanes 3-5 and 7-9 as formed by nuclear extracts from PM and MA muscle fibers either innervated or incubated in medium containing ryanodine. A faster migrating protein-DNA complex (asterisk) of unknown composition was present in each lane. (B) Inclusion of anti-MEF2A antibody in the binding reaction caused the formation of a supershifted complex (arrow). Inclusion of a NFATc1 antibody did not decrease the mobility of the MEF2A-containing complex.





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