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Fig. 5. Endogenous 4.1R is present in isolated centrosomes. (A) Centrosomes isolated from Molt-4 T cells were subjected to double immunofluorescence with anti-4.1R (4.1R) and anti- ${gamma}$ -tubulin ( ${gamma}$ -tubulin) or anti- ${alpha}$ -tubulin ( ${alpha}$ -tubulin) antibodies. The samples were analysed by epifluorescence microscopy. (B) Western-blot analyses of isolated centrosomes were carried out with anti-4.1R (4.1R), anti- ${gamma}$ -tubulin ( ${gamma}$ -tubulin) and anti-actin (actin) antibodies. (C) In-vitro-assembled microtubule asters were triple stained with anti- ${alpha}$ -tubulin ( ${alpha}$ -tubulin), anti-4.1R (4.1R) and anti- ${gamma}$ -tubulin ( ${gamma}$ -tubulin) antibodies. The images are projections of optical-section stacks acquired by confocal microscopy. Bar, 10 µm.

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